Background & Aims: Patient safety is the priority of the operating room. Since retained surgical items and specimen management errors are the most important safety issues, operating room nurses and technologist are in a unique position to report the errors that occur in the OR. On the other hand, documentation is also the task of these personnel, so the aim of present study is to determine the effect of counting surgical items and specimen care documentation forms on the number of reported errors. Materials & Methods: A semi-experimental, single-group and pre-post design was conducted. Sampling was done by census and 65 technologists and nurses met the study inclusion criteria. The data collection tool was an error reporting questionnaire. During intervention documentation of the intraoperative care was performed for two months and the error reporting questionnaire was distributed before and after intervention period. Results: The frequency of unreported errors after the intervention was significantly decreased but the frequency of oral reported errors were not significantly different between before and after the intervention, and the frequency of counting surgical items written errors after the intervention was significantly increased. Conclusion: Using appropriate documentation can encourage OR nurses and technologists to report errors and increase patient safety.

In forensic DNA analysis of living individuals, blood and buccal swabs are commonly used as sources of genetic material. While blood collection requires an invasive procedure that may cause discomfort, buccal swabs provide a non-invasive and painless alternative for obtaining DNA samples. However, due to Indonesia’s geographical landscape, the samples delivery to genetic laboratories often involves extended transit times. This study aims to evaluate the quality of DNA obtained from buccal swabs by comparing results immediately after extraction with those obtained after storage. The average DNA purity (A260/A280) remained relatively stable from day 0 to day 14, ranging from 1.75 to 1.96. DNA concentration showed slight fluctuations, with the highest value recorded on day 14 (597.70 micrograms per milliliter) and the lowest on day 7 (516.90 micrograms per milliliter). No significant variations were observed in either DNA purity or concentration over time. The findings of this study demonstrate that DNA extracted from buccal swabs maintains optimal quality for up to 14 days of storage, provided optimal storage conditions are followed. This suggests that buccal swab DNA exhibits stable characteristics over a defined period, supporting its use in different genetic analyses without significant degradation.